Hypoglycemic activity of Phaseolus vulgaris (L.) aqueous extract in type 1 diabetic rats
Curr Issues Pharm Med Sci., Vol. 32, No. 4, 210-218
Tetiana Halenova1*, Natalia Raksha1, Olha Kravchenko1, Tetiana Vovk1,
Alona Yurchenko1, Igor Vareniuk2, Olexii Savchuk1, Ludmila Ostapchenko1
1 Department of Biochemistry Educational an d Scientific Center “Institute of Biology and Medicine” Taras Shevchenko National University of Kyiv, 64/13 Volodymyrska Street, 01601 Kyiv, Ukraine
2 Department of Cytology Educational and Scientific Center “Institute of Biology and Medicine” Taras Shevchenko National University of Kyiv, 64/13 Volodymyrska Street, 01601 Kyiv, Ukraine
The aim of the present study was to evaluate the hypoglycemic activity of the aqueous extract from the fruit walls of Phaseolus vulgaris pods and to examine the potential mechanism underlying the improvement of the glycemic level. In the course of the study, diabetes mellitus was induced in rats with a single intraperitoneal injection of streptozotocin (45 mg·kg-1 b.w.). Diabetic and control rats were then orally administered with a single-dose or repeated-dose (28 day) of P. vulgaris extract (200 mg·kg-1). Results show that the extract was found to possess significant hypoglycemic activity, and the study of glucose utilization by isolated rat hemidiaphragm suggests that the aqueous extract may enhance the peripheral utilization of glucose. The subsequent experiments have revealed that the P. vulgaris extract could increase glucose transporter 4 (GLUT-4) content in skeletal muscle cells of control and diabetic rats. Our data also indicate that the P. vulgaris extract did not affect the content of the insulin receptor, but significantly reduced the total tyrosine kinase activity in skeletal muscle cells of both experimental groups of rats. The present results clearly indicated that P. vulgaris extract may be beneficial for reducing hyperglycemia through its potency in regulation of glucose utilization via GLUT-4, but the current mechanism remains to be unidentified.