Current Issues of Pharmacy and Medical Sciences

Obtaining the tbf gene which encodes immunodominant epitopes of pathogenic cholera strains

Curr Issues Pharm Med Sci., Vol. 32, No. 3, 130-133

Yulia Bazarnova*, Tatiana Bolotnikova, Ekaterina Aronova

Higher School of Biotechnology and Food Technology, Peter the Great Saint Petersburg State Polytechnical University 


https://doi.org/10.2478/cipms-2019-0024

Abstract

We experimentally carried out the synthesis of the tbf gene that encodes highly-immunogenic epitopes of pathogenic cholera strains, through the cloning of the tbf gene in pGEM-T Easy plasmid. Moreover, we tested the hybrid gene sequence for absence of mutations, using the Sanger sequencing. We also calculated the nucleatic sequence of the tbf gene. The obtained results have both scientific and practical significance.

Keywords

candidate cholera vaccine, hybrid tbf gene, Sanger sequencing, protein design, TBF recombinant protein, immunodominant epitopes, Vibrio choleraе.

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