Current Issues of Pharmacy and Medical Sciences

The development of HPLC-DAD method for determination of active pharmaceutical ingredient in the potassium 2-((4-amino-5-(morpholinomethyl)-4H-1,2,4-triazol-3-yl)thio)acetate substance

Curr Issues Pharm Med Sci., Vol. 32, No. 1, 5-9

Roman Shcherbyna1, Volodymyr Parchenko1
Boris Varynskyi2, Andriy Kaplaushenko2

1 Department of Toxicological and Inorganic Chemistry, Zaporizhzhya State Medical University, Mayakovsky 26, 69035, Zaporizhzhya, Ukraine
2 Department of Physical and Colloidal Chemistry, Zaporizhzhya State Medical University, Mayakovsky 26, 69035, Zaporizhzhya, Ukraine


https://doi.org/10.2478/cipms-2019-0001

Abstract

Derivatives of 1,2,4-triazole are actively researched by scientists and synthetic pharmacologists. The last studies have shown that potassium 2-((4-amino-5-(morpholinomethyl)-4H-1,2,4-triazol-3-yl)thio)acetate with low toxicity series exhibits antioxidant and hepatoprotective properties. Therefore, the purpose of this work was to develop a method for determining the API in the potassium 2-((4-amino-5-(morpholinomethyl)-4H-1,2,4-triazol-3-yl)thio)acetate substance using the method of high-performance liquid chromatography with diode array detection (HPLC-DAD). As a result of this work, it is shown that the developed method is specific and meets the requirements of linearity, accuracy and precision. The results of determining the contents of the API in real samples indicate that the method can be proposed to control the quality of the potassium 2-((4-amino-5-(morpholinomethyl)-4H-1,2,4-triazol-3-yl)thio)acetate substance.

Keywords

1, 2, 4-triazole, HPLC-DAD, an active pharmaceutical ingredient.

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