Current Issues of Pharmacy and Medical Sciences

A comparative study of phenolic compound antioxidant activity by the polarography method, using microsomal lipid peroxidation in vitro

Curr Issues Pharm Med Sci., Vol. 31, No. 4, 186-189

Anatolii Gordiienko1, Mykola Blazheyevskyi2, Ivan Iurchenko3

1 Department of Pharmacology and Toxicology, Kharkiv State Zooveterinary Academy, 62341, Kharkiv Region, Dergachiv District, Mala Danylivka, Ukraine 
2 Department of Physical and Colloidal Chemstry, National University of Pharmacy, 61168, Kharkiv, Valentynivska 4, Ukraine
3 Department of Physcolloidal Chemistry, Zaporozhye State Medical University, 69035, Zaporizhzhia, Maiakovskyi ave 26, Ukraine

DOI: 10.1515/cipms-2018-0034


For comparative purposes, a quantitative estimation of antioxidant activity of phenolic compounds of different classes was conducted by way of the polarography method, via the ADP-Fe2+ model of the induced ascorbate-dependent lipid peroxidation of rat liver microsomes within an in-vitro system. As a result, it was recognized that the antioxidant properties of phenolic compounds depend on the nature and chemical structure of several substances. In respect of such activity, leaders in the classes of investigated polyphenolic compounds are: Propyl gallate = Gallotannin (Phenolcarboxylic acids and their derivatives) > Quercetin = Myricetin (Flavonols) > Luteolin (Flavo n) = Mangiferin (Xanthones) > Kaempferol (Flavonols) = Catechin (Flavans). Thus, the assessment of the inhibition ability of the lipid peroxidation of microsomes by phenolic compounds can be used as an accessible test for the preliminary quantitative estimation of their antioxidant properties.


lipid peroxidation of microsomes, antioxidant activity, phenolic compounds, polarography method.


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