Current Issues of Pharmacy and Medical Sciences

Chemical composition, toxicity, and acaricidal activity of Eucalyptus globulus essential oil from Algeria

Curr Issues Pharm Med Sci., Vol. 31, No. 2, 89-93

Ghania Atmani-Merabet1,2,3*, Abdelmalik Belkhiri1,2, Mohamed Abdeslam Dems4,
Abdeldjallil Lalaouna1, Zakaria Khalfaoui5, Bouzid Mosbah6

Departments of Dental Surgery and Pharmacy, Laboratory of Pharmacognosy, Salah Boubnider University Constantine 3, Algeria
2 Laboratory of Pharmacology and Toxicology, Mentouri University Constantine 1, Algeria
3 Departments of Chemistry, Faculty of Sciences, Mentouri University Constantine 1, Algeria
4 Biotechnology Research Centre (Crbt Constantine), Constantine, Algeria
5 Technical Institute of Breeding (ITE Hamma Bouziane), Constantine, Algeria
6 Forest Conservation in Constantine, Algeria

DOI: 10.1515/cipms-2018-0017 10.1515/cipms-2018-0011


The study was aimed at determining the chemical composition, toxicity effect and field varroacidal efficacy of the essential oil distilled from the leaf of Eucalyptus globulus (Eg) grown in Algeria. Brine shrimp lethality (BSL) assay and bee hives infected by Varroa destructor were used to assess the toxicity and acaricidal effect, respectively. Steam distillation of leaves yielded 0.93% (v/w) of essential oil (EO). GC/MS Analysis revealed 39 compounds, essentially oxygenated monoterpenes (86.01%). The main constituents of the oil were 1,8-cineole (78.45%), o-cymene (2.18%), isopinocarveole (1.74%), α-pinene (1.69%), pinocarvone (1.34%) and veridiflorol (1.31%). The BSL assay revealed a highly toxic value of LC50 (67.55 µg/mL). Furthermore, field efficacy testing on bee hives infected with Varroa destructor has confirmed the effectiveness of Eucalyptus globulus essential oil (EgEO) or thymol as varroacidal agents. Moreover, a EgEO + thymol association was more effective than EgEO or thymol alone. Finally, the use of EgEO + thymol may constitute a viable alternative to the thymol-based commercial treatment.

Full text


Eucalyptus globulus, leaf essential oil, thymol, toxicity, Varroa destructor



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