Current Issues of Pharmacy and Medical Sciences

The fluctuation of free amino acids in serum during acute ischemic stroke

Curr Issues Pharm Med Sci., Vol. 29, No. 4, 151-154

Maria Szpetnar1, Anna Hordyjewska1, Iwona Malinowska2,
Piotr Golab3, Jacek Kurzepa1

1 Department of Medical Chemistry, Medical University of Lublin, Chodzki 4a, 20-093 Lublin, Poland
Department of Mathematics, Lublin University of Technology, Lublin, Poland
Department of Internal Medicine, The Holy Family Specialist Hospital in Rudna Mala, Poland

DOI: 10.1515/cipms-2016-0021 

FULL TEXT

Abstract

Currently, little data exists regarding the involvement of free amino acids (AA) in the pathogenesis of ischemic stroke (IS). Thus, our objective was to study the degree of the degree of fluctuation of free amino acids level in serum during the acute phase of IS. The study consisted of eighteen patients (female/male: 10/8; age: 73.1 ± 4.1) with acute IS that was confirmed by way of computed tomography, while twelve sex and age matched individuals were assigned as control group. During the study period, the patients did not receive any supplemental amino acids therapy that could affect the obtained results. The venous blood was obtained after >3 hours fasting at two time-points; time-point 1 – at admission to the hospital; time-point 2 – on day 5 from stroke onset. The blood for control purposes was collected only once, and the blood collection at time-point 1 was done before thrombolytic treatment (nine patients). The amino acids were identified using the Amino Acids Analyser (AAA 400) by INGOS Corp., Praha, Czech Republic. Our results revealed a statistically significant increase of glutamate, cystine and methionine on day 1 of stroke, in comparison to control, whereas, proline level was decreased on day 1 of stroke – in comparison to control serum. On comparing day 5 to the initial day of IS, elevation was observed of levels of asparagine, glycine, tyrosine, arginine, threonine, valine, leucine and phenylalanine. It can be said, then, that ischemic stroke induces both essential and nonessential amino acid fluctuations. Moreover, the decrease in proline and glutamine serum level with the simultaneous increase in the concentration of branch chain amino acids, Glu and Thr suggests a violent mobilization of the body’s proteins. Thus, a decrease of Pro and a simultaneous increase of Glu serum level could be considered as a marker of acute IS.

Keywords

amino acids, biomarker, ischemic stroke.

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