Current Issues of Pharmacy and Medical Sciences

Simultaneous determination of amlodipine and telmisartan from pharmaceutical products by way of capillary electrophoresis

Curr Issues Pharm Med Sci., Vol. 29, No. 1, p.42-45

Modroiu Adriana, Hancu Gabriel*, Vlad Robert Alexandru, Stăcescu Ștefana, Soare Ruxandra, Kelemen Hajnal

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy Tîrgu Mureş, Gh. Marinescu 38, 540139 Tîrgu Mureş, Romania

DOI: 10.1515/cipms-2016-0010

 

Abstract

A rapid, simple and sensitive capillary zone electrophoresis method was developed for the simultaneous determination of amlodipine besylate and telmisartan, in fixed-dose combinations, through the utilization of a UV photodiode array detector. Electrophoretic parameters such as buffer concentration and pH, system temperature, applied voltage and injection parameters, were optimized in order to improve the efficiency of the separation. The best results were obtained when employing fused silica capillary (48 cm length X 50µm ID) and 50 mM phosphate buffer electrolyte at pH 4.50, + 25 kV applied voltage, as well as 25°C system temperature. The separation was achieved in approximately 3 minutes, with a resolution of 4.90, while the order of migration was amlodipine followed by telmisartan. The analytical performance of the method was verified with regard to linearity, precision and robustness. In addition, the limit of detection and the quantification were calculated.

 

FULL TEXT

Keywords

fixed dose combinations, Amlodipine, Telmisartan, Capillary electrophoresis, Simultaneous determination.

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