Synergistic interaction of levetiracetam with gabapentin in the mouse 6 Hz psychomotor seizure model ...
Curr Issues Pharm Med Sci., Vol. 28, No. 3, Pages 204-207
Aleksandra Wlaz, Maria W. Kondrat-Wrobel, Katarzyna Zaluska,
Ewelina Kochman, Anna R. Rekas, Jarogniew J. Luszczki
1 Department of Pathophysiology, Medical University, Jaczewskiego 8, 20-090 Lublin, Poland
2 Isobolographic Analysis Laboratory, Institute of Rural Health, Jaczewskiego 2, 20-090 Lublin, Poland
This study was aimed at characterizing the anticonvulsant effects of levetiracetam in combination with gabapentin, in the mouse 6 Hz psychomotor seizure model. Herein, psychomotor seizures were evoked in male albino Swiss mice by a current (32 mA, 6 Hz, 3 s stimulus duration) delivered via ocular electrodes. Type II isobolographic analysis was used to characterize the anticonvulsant interactions between the drugs in combination, for fixed-ratios of 1:1, 1:2, 1:5 and 1:10. The type II isobolographic analysis revealed that the combinations of levetiracetam with gabapentin for the fixed-ratios of 1:5 and 1:10 were supra-additive (synergistic; P<0.05) in terms of seizure suppression, while the combinations for the fixed-ratios of 1:1 and 1:2 were additive in the mouse 6 Hz psychomotor seizure model. We conclude that, as the combinations of levetiracetam with gabapentin for the fixed-ratios of 1:5 and 1:10 exerted supra-additive (synergistic) interaction in the mouse 6 Hz psychomotor seizure model, this may be considered as particularly favorable combinations in further clinical practice.
6 Hz psychomotor seizure model, antiepileptic drugs, drug interactions, gabapentin, levetiracetam, isobolographic analysis.