Current Issues of Pharmacy and Medical Sciences

Designing primers potentially specific to Entamoeba gingivalis genes

Curr Issues Pharm Med Sci., Vol. 28, No. 3, Pages 159-163

Jagoda Abramek

Chair and Department of Biology and Genetics, Faculty of Pharmacy; Medical University of Lublin; Chodzki 4a, 20-093 Lublin, Poland


DOI: 10.1515/cipms-2015-0064

Abstract

Entamoeba gingivalis normally exists in the human oral cavity, namely in the gums, and brings about some specific diseases. However, it can also trigger some more serious illnesses. Among these are infections of the genital tract, acute osteomyelitis of the mandible and pulmonary abscess. Entamoeba gingivalis identification by light microscopy is difficult, hence polymerase chain reaction (PCR) is used. The contemporary primers for PCR are complement to 18S rRNA. This article informs the reader of the process that was involved in designing new primers for three genes which were thought to be present on the Entamoeba gingivalis genome, but their sequences were unknown. The newly obtained sequences of primers have better properties for identification purposes, compared to these which are currently used.

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Keywords

actin, cysteine proteinase, Entamoeba gingivalis, 18S-rRNA, 5.8S-rRNA, PCR primers.

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