Current Issues of Pharmacy and Medical Sciences

The alteration in peripheral neutrophils of patients with chronic kidney disease

Curr Issues Pharm Med Sci., Vol. 28, No. 1, Pages 17-20

Larissa Yevgenyevna Muravlyova*, Vilen Borisovich Molotov-Luchankiy,
Ryszhan Yemelyevna Bakirova, Anar Akylbekovna Turmukhambetova,
Dmitriy Anatolyevich Klyuyev, Ludmila Andreevna Demidchik,
Yevgeniya Alexandrovna Kolesnikova

Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan

DOI: 10.1515/cipms-2015-0034

Abstract

Recent findings have demonstrated the impaired functions of neutrophils of patients with chronic renal failure. The purpose of our research was to study oxidative modified proteins, as well as the histone spectrum in neutrophils drawn from patients with chronic kidney disease, and to estimate the ability of neutrophils to form spontaneous neutrophil extracellular traps. In this work, we have assumed that metabolic alteration in neutrophils may develop at early stages of chronic kidney disease. Materials and methods: Neutrophils obtained from patients with various stages of chronic kidney disease and degrees of chronic renal failure were used. As control, blood samples obtained from 32 healthy individuals was employed. In the examined neutrophils, advanced oxidation protein products, protein reactive carbonyl derivatives, as well as nucleosomal histones were detected. The ability of neutrophils to form spontaneous neutrophil extracellular traps was estimated. Our results have demonstrated an increase of nucleosomal histones in neutrophils of all patients with chronic kidney disease. Moreover, our work fixes the rate of growth of intracellular advanced oxidation protein products and the decreasing of protein reactive carbonyl derivatives in neutrophils from patients with chronic kidney disease. Furthermore, we demonstrate the presence of the neutrophils with altered oxidative status and the decomposition of the histone spectrum in the circulation of patients with chronic kidney disease.

 

Full Text

Keywords

neutrophils, oxidative proteins, histones, neutrophils extracellular traps chronic kidney disease.

Calendar

April 2020

Mon Tue Wed Thu Fri Sat Sun
    01 02 03 04 05
06 07 08 09 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30