Current Issues of Pharmacy and Medical Sciences

Comparative validation of HPLC, densitometric and videodensitometric determination of lamotrigine in pharmaceutical

Curr Issues Pharm Med Sci., Vol. 27, No. 4, Pages 258-262

Beata Paw

Chair and Department of Medicinal Chemistry, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland

DOI: 10.1515/cipms-2015-0029


Simple, sensitive, precise and accurate HPLC, densitometric and videodensitometric methods for determination of lamotrigine in tablet forms were developed and validated. The HPLC method was carried out using a Symmetry C8 column and a mobile phase acetonitrile-phosphate buffer pH 2.80 (25:75, v/v), with a flow rate of 1 mL/min, and UV detection at 210 nm. Ethosuximide was used as the internal standard. Densitometric and videodensitometric analysis was performed on silica gel 60 F254 plates, in horizontal chambers, with methanol-chloroform-ammonia (25%) 1.5:7.5:1, (v/v) as mobile phase. Densitometric detection was performed at 225 nm and at 315 nm, and videodeoscanning at 254 nm. Calibration plots were constructed in the range 0.5-10 µg/spot, with good correlation coefficients r > 0.99 for both methods. The precision and accuracy of all elaborated methods were compared. Finally, the developed methods were applied for the quality control of lamotrigine tablets.




densitometry, videodensitometry, lamotrigine, tablets


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