Current Issues of Pharmacy and Medical Sciences

Determination of caffeic acid in root and rhizome of Black cohosh (Cimicifuga racemosa (L.) Nutt).

Curr Issues Pharm Med Sci., Vol. 27, No. 2, Pages 108-110

Karolina Zapala1, Grazyna Szymczak2, Magdalena Wojciak-Kosior1

1 Department of Analytical Chemistry, Medical University of Lublin, Chodzki 4a, 20-093, Lublin, Poland
2 Botanical Garden of Maria Curie-Skłodowska University in Lublin, Slawinkowska 3, 20-810 Lublin, Poland

DOI: 10.2478/cipms-2014-0025



Cimicifuga racemosa, is a plant with a diverse and long history of medicinal use. Caffeic acid, bioactive compound, which often occurs with other polyphenols can influence the biological activity of this plant. The aim of our work was quantitative analysis of caffeic acid in roots and rhizomes of two varieties of C. racemosa. Analysis was performed by HPLC method. The extracts were separated on C18 reversed-phase column using mixture of methanol, water and formic acid (25:75:0.5 v/v/v) as a mobile phase. The flow rate of eluent was 1.0 ml·min-1. The obtained validation parameters such as linearity, linear regression equation and precision expressed as a relative standard deviation were adequate for quantitative determination. Caffeic acid was found in all tested extracts. The highest total amount of caffeic acid was determined in C. racemosa var. racemosa (255.3 µg·g-1) while its concentration in C. racemosa var. cordifolia was significantly lower (213.0 µg·g-1).


Full text


caffeic acid, Cimicifuga racemosa, phenolic acids


  1. Anwar J. et al.: Effects of caffeic acid on behavioral parameters and on the activity of acetylcholinesterase in different tissues from adult rats. Pharmacol. Biochem. Behav., 103, 386, 2012.
  2. Gülçin Ï.: Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicology 217, 213, 2006.
  3. Hui Z. et al.: Effects of black cohosh and estrogen on the hypothalamic nuclei of ovariectomized rats at different temperatures. J. Ethnopharmacol. 142, 769, 2012.
  4. Jiang B. et al.: The value of plant collections in ethnopharmacology: a case study of an 85-year-old black cohosh (Actaea racemosa L.) sample. J. Ethnopharmacol. 96, 521, 2005.
  5. Khan A.Q. et al.: Caffeic acid attenuates 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced NF- κB and COX-2 expression in mouse skin: abrogation of oxidativestress, inflammatory responses and proinflammatory cytokine production. Food Chem. Toxicol. 50, 175, 2012.
  6. Leite F. R. F., Santos W. de J. R., Kubota L. T.: Selective determination of caffeic acid in wines with electrochemical sensor based on molecularly imprinted siloxanes. Sensors and Actuators B: Chemical 193, 238, 2014.
  7. Low Dog T., Powell K.L., Weisman S.M.: Critical evaluation of the safety of Cimicifuga racemosa in menopause symptom relief. Menopause 10, 299, 2003.
  8. Margaret R.: Alternative treatments for the menopause. Clin. Obstet. Gynecol. 23, 151, 2009.
  9. Naczk M., Shahidi F.: Exctraction and analysis of phenolics in food. J. Chromatogr. A 1054, 95, 2004.
  10. Oh J. et al.: Antioxidant and antimicrobial activities of various leafy herbal teas. Food Control 31, 403, 2013.
  11. Tomás-Barberán F.A. et al.: HPLC - DAD - ESIMS analysis of phenolic compounds in nectarines, peaches, and plums. J. Agric. and Food Chem. 49, 4748, 2001.
  12. Weng C., Yen G.: Chemopreventive effects of dietary phytochemicals against cancer invasion and metastasis: phenolic acids, monophenol, polyphenol, and their derivatives. Cancer Treat. Rev. 38, 76, 2012.
  13. Xie Y. et al.: Caffeic acid derivatives: A new type of influenza neuraminidase inhibitors. Bioorg. Med. Chem. 23, 3556, 2013.


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