Current Issues of Pharmacy and Medical Sciences

Effect of N-(m-bromoanilinomethyl)-p-isopropoxyphenylsuccinimide on the anticonvulsant action of four classical antiepileptic ..

Curr Issues Pharm Med Sci., Vol. 27, No. 2, Pages 76-79

Jarogniew J. Luszczki 1,2,*, Ewa Marzeda 1, Maria W. Kondrat-Wrobel 2, Daniel Pyrka 2, Sergey L. Kocharov 3, Magdalena Florek-Luszczki 4

1 Isobolographic Analysis Laboratory, Institute of Rural Health, Jaczewskiego 2, 20-950 Lublin, Poland
2 Department of Pathophysiology, Medical University of Lublin, Ceramiczna 1, 20-150 Lublin, Poland
3 Mndjoyan’s Institute of Fine Organic Chemistry, National Academy of Sciences, Azatutyan Avenue 26, 375014 Yerevan, Republic of Armenia
4 Department of Public Health, Institute of Rural Health, Jaczewskiego 2, 20-950 Lublin, Poland

DOI: 10.2478/cipms-2014-0017

 

Abstract

Effect of N-(m-bromoanilinomethyl)-p-isopropoxyphenylsuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model

 

The purpose of this study was to determine the effects of N-(m-bromoanilinomethyl)-p-isopropoxyphenylsuccinimide (BAM-IPPS – a new succinimide derivative) on the protective action of four classical antiepileptic drugs (AEDs: carbamazepine [CBZ], phenobarbital [PB], phenytoin [PHT] and valproate [VPA]) in the mouse maximal electroshock (MES)-induced tonic seizure model. Tonic hind limb extension (seizure activity) was evoked in adult male albino Swiss mice by a current (sine-wave, 25 mA, 500 V, 50 Hz, 0.2 s stimulus duration) delivered via ear-clip electrodes. BAM-IPPS administered (i.p.) at a dose of 150 mg/kg significantly elevated the threshold for electroconvulsions in mice (P<0.05). Lower doses of BAM-IPPS (50 and 100 mg/kg) had no significant impact on the threshold for electroconvulsions in mice. Moreover, BAM-IPPS (100 mg/kg) did not significantly affect the anticonvulsant potency of CBZ, PB, PHT and VPA in the mouse MES model. BAM-IPPS elevated the threshold for electroconvulsions in mice in a dose-dependent manner. However, BAM-IPPS (100 mg/kg) did not affect the anticonvulsant action of various classical AEDs in the mouse MES model, making the combinations of BAM-IPPS with CBZ, PB, PHT and VPA neutral, from a preclinical point of view.

 

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Keywords

antiepileptic drugs, maximal electroshock-induced seizures, N-(m-bromoanilino-methyl)-p-isopropoxyphenylsuccinimide

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