Current Issues of Pharmacy and Medical Sciences

In vitro evaluation of immunogenic properties of active dressings

Curr Issues Pharm Med Sci., Vol. 27, No. 1, Pages 55-60

Joanna Orłowska¹,³*, Urszula Kurczewska², Katarzyna Derwińska³,
Wojciech Orłowski³, Daria Orszulak-Michalak²

¹ Ph.D. Student, Medical University of Lodz, 1 Pl. Hallera St., 90-647 Lodz, Poland
² Department of Biopharmaceutics, Medical University of Lodz, 1 Muszyńskiego St., 90-153 Lodz, Poland
³ Science and Research Laboratory Dermin Sp. z o.o., 23 Milionowa St., 93-193 Lodz, Poland

DOI: 10.2478/cipms-2014-0014

 

Abstract

The aim of this study was in vitro evaluation of the level of the immune response in relation to wound dressings composed of alginate, calcium carboxymethylcellulose, and dibutyrylochitin and determination of the direction of response, which will make referring next to the results of in vivo phase possible.
The subject of the experiments was to examine the commercially available, biodegradable alginate dressing, commercially available but not biodegradable dressing constructed from the sodium carboxymethylcellulose, and synthesized in house biodegradable dressing constructed of the dibutyrylchitin. To determine the direction of the immune response, the degree of secretion of pro-inflammatory interleukin (IL-1, IL-6) and anti-inflammatory (IL-10) interleukin from murine fibroblasts having contact with the tested dressings (ELISA enzyme linked immunosorbent assay), was tested.
 

Keywords

dressings, immune response, inflammation

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