Essential oil composition and variability of Hypericum perforatum L. from wild population in Kosovo
Curr Issues Pharm Med Sci., Vol. 27, No. 1, Pages 51-54
Avni Hajdari1*, Behxhet Mustafa1, Dashnor Nebija2, Albana Kashtanjeva1,
Jarosław Widelski3, Kazimierz Głowniak3, Johannes Novakd4
Aerial parts of Hypericum perforatum L. (Hypericaceae) were collected from five wild populations in Kosovo, with aim to investigate the chemical composition and natural variation of essential oils between wild populations. This species could be considered of economic potential as it is widespread in Kosovo, on the other hand H. perforatum is one of the best-known medicinal herbs used in Kosovo folk medicine. Essential oils were obtained by steam distillation and analysed by GC-FID and GC-MS. Sixty-seven components were identified. The yields of essential oils differed depending on the population and ranged from 0.04 to 0.26% based on dry weight. The aerial parts of H. perforatum were characterized by the following main constituents: 2-methyl-octane (1.1-15.5%), α-pinene (3.7-36.5%), β-caryophyllene (1.2-12.4%), caryophyllene oxide (3.3-17.7%) and n-tetradecanol (3.6-10.4%). Hierarchical cluster analysis revealed that the concentration of components depends on the origin of the plant populations, thus α-pinene and 2-methyl-octane were present in the highest concentration in population originating from Gjakovë, Prizren and Ferizaj, whereas in the populations originating from Pejë and Prishtinë the most abundant constituents were caryophyllene oxide, β-caryophyllene and n-tetradecanol. Further investigation is needed to establish the natural variability and chemopolymorphism of this species in the territory of Kosovo, which should be supported by molecular level analyses.
- Adams RP. (2009). Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. 4th Edn., Allured Publishers, Carol Stream, IL., USA.
- Baser K.H.C., Ozek T., Nuriddinov H.R., Demirci A.B.: Essential oil of two Hypericum species from Uzbekistan. Chem. Nat. Compd., 38:1, 54-57, 2002.
- Bradley, P. British Herbal Compendium. Vol 2. British Herbal Medicin Association, 2006.
- Cakir A., Duru M.E., Harmandar M., Ciriminna R., Passannanti S., Piozzi F., Comparison of the volatile oils of Hypericum scabrum L. and Hypericum perforatumin Turkey. Flavour Fragrance J., 12, 285-287, 1997.
- Crockett S.L., Robson N.K.: Taxonomy and chemotaxonomy of the genus Hypericum Med. Aromat. Plant Sci. Biotechnol., 5, 1-13, 2011.
- Erken S., Malyer H., Demirci F., Demirci B., Baser K.H.C., Chemical investigations on some Hypericumspecies growing in Turkey-I. Chem. Nat. Compd., 37, 434-438, 2002.
- European Pharmacopeia. (2011). European Pharmacopoeia. 6th Edn., Maisonneuve Publishers, Strasbourg, France.
- Hobbs C.: St. John’s wort, Hypericum perforatum L. Herbalgram, 18, 24-33, 1989.
- Mahady G.B., Fong H.H.S., Farnsworth N.R. (2001). WHO monographs of selected medicinal plants II. World Health Organization, Geneva, Switzerland.10. Mimica-Dukic N., Ivancev-Tumbas I., Igic R., Popovic M., Gasic O.: The content and composition of essential oil of Hypericum perforatumfrom Serbia. Pharm. Pharmacol. Lett., 8, 26-28, 1997.
- Mustafa B., Hajdari A., Krasniqi F., Hoxha E., Ademi H., Quave C.L., Pieroni A., Medical ethnobotany of the Albanian Alps in Kosovo. J. Ethnobiol. Ethnomed., 8:6, 1-14, 2012.
- Mustafa B., Hajdari A., Pajazita Q., Syla B., Quave C.L., Pieroni A., An ethnobotanical survey of the Gollak region, Kosovo. Gen. Resour. Crop Evol., 59, 739-754, 2012.
- Nahrstedt A, Butterweck V. Biologically active and other chemical constituents of the herb of Hypericum perforatum L. Pharmacopsychiatry, 30, 129-134, 1997.
- Schwob I., Bessiere J.M., Viano, J. Composition of the essential oils of Hypericum perforatum L. from Southeastern France. C.R. Biologies, 325, 781-785, 2002.
- Smelcerovic A., Spiteller M., Ligon A.P., Smelcerovic Z., Raabe N.: Essential oil composition of Hypericum L. species from Southeastern Serbia and their chemotaxonomy. Biochem. Syst. Ecol., 35, 99-113, 2007.
- Tutin G.T., Haywood H.V., Burges A.N., Moore D.M., Valentine D.H., Walters S.M., Webb D.A. (1972) Flora Europea. Vol. 3, Cambridge University Press, Cambridge, UK.
- Vasileva R., Georgieva V., Vojnova J., Milkova T.: GC-MS study on the essential oils of Bulgarian Hypericum maculatum Grantz and Hypericum perforatum L. Comptes Rendus Academie Bulgare Sciences, 56, 71-74, 2003.
- Wichtl M., Teedrogen. Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart, 4. Auflage 2002.