Current Issues of Pharmacy and Medical Sciences

The inhibition of rat leukocytes apoptosis under the condition of experimental diabetes mellitus type 1 by Galega officinalis L.

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 4, Pages 393-397

MARIIA KHOKHLA, GALYNA KLEVETA, MARJANA LUPAK, MARIIA SKYBITSKA,
YAROSLAV CHAJKA, NATALIA SYBIRNA

Ivan Franko National University of Lviv, Biochemistry Department, Ukraine

DOI: 10.12923/j.2084-980X/26.4/a.09

 

Abstract

The article contains the data of Galega officinalis extract (GOE) nonalkaloid-containing fraction influence on the structural and functional state of leukocytes under the condition of experimental diabetes mellitus type 1 (EDM). Immunohistochemical method has shown the quantitative redistribution of leukocytes containing proapoptotic protein p53 and PARylated [poly(ADP-ribosyl) ated] proteins under EDM conditions and admission of the tested extract. GOE administration to animals with EDM leads to the reduction in the number of cells with positive and strongly positive reaction to the p53 protein and PARylated proteins content, indicating its depressing effect on apoptosis of immune cells.

Keywords

Galega officinalis L., diabetes mellitus, leukocytes, apoptosis, sialic acid

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