Current Issues of Pharmacy and Medical Sciences

The inhibition of rat leukocytes apoptosis under the condition of experimental diabetes mellitus type 1 by Galega officinalis L.

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 4, Pages 393-397


Ivan Franko National University of Lviv, Biochemistry Department, Ukraine

DOI: 10.12923/j.2084-980X/26.4/a.09



The article contains the data of Galega officinalis extract (GOE) nonalkaloid-containing fraction influence on the structural and functional state of leukocytes under the condition of experimental diabetes mellitus type 1 (EDM). Immunohistochemical method has shown the quantitative redistribution of leukocytes containing proapoptotic protein p53 and PARylated [poly(ADP-ribosyl) ated] proteins under EDM conditions and admission of the tested extract. GOE administration to animals with EDM leads to the reduction in the number of cells with positive and strongly positive reaction to the p53 protein and PARylated proteins content, indicating its depressing effect on apoptosis of immune cells.


Galega officinalis L., diabetes mellitus, leukocytes, apoptosis, sialic acid


  1. Azuma Y., Taniguchi A., Matsumoto K.: Decrease in cell surface sialic acid in etoposide-treated Jurkat cells and the role of cell surface sialidase. Glycoconj. J., 17, 301, 2000.
  2. Aksenov D.V. et al: Effect of plant extracts on trans-sialidase activity in human blood plasma. Bull Exp Biol Med., 143 (1), 52, 2007.
  3. Braithwaite A., Royds J., Jackson P.: The p53 story: layers of complexity. Carcinogenesis., 26, 1161, 2005.
  4. Briehl M.M., Cotgreave I.A., Powis G.: Downregulation of the antioxidant defence during glucocorticoid-mediated apoptosis. Cell Death Differ., 2, 41, 1995.
  5. Drel V., Sybirna N.: Protective effects of polyphenolics in red wine on diabetes associated oxidative/nitrative stress in streptozotocindiabetic rats. Cell Biol. Int., 34, 1147, 2010.
  6. Jazayeri L. et al.:  Diabetes increases p53-mediated apoptosis following ischemia. Plastic and Rec. Surg., 121, 1135, 2008.
  7. Haden G. Goat’s rue – French lilac – Italic Fitch – Spanish scifoin: galega officinalis and metformin. Royal college of Edinburg., 35, 258, 2003.
  8. Hetts S.W.: To die or not to die: An overview of apoptosis and its role in disease. JAMA, 279, 300, 1998.
  9. Kiselova Y. et al.: Polyphenol content and in vitro antioxidant activity of aqueus-alcohoholic extracts from Bulgarian herbs. Bull. Med. Inst. after Mehrabyan., 1, 78, 2006.
  10. Khokhla M. R. et al.: Cytological and biochemical characteristics of rats’ peripheral blood under the condition of experimental diabetes mellitus type 1 and Galega officinalis admission. Studia Biologica, 6(1), 37, 2012.
  11. Malanga M., Althaus F.R.: The role of poly(ADP-ribose) in the DNA damage signaling network. Biochem. Cell. Biol., 83, 354, 2005.
  12. Meesmann H.M. et al.: Decrease of sialic acid residues as an eat-me signal on the surface of apoptotic lymphocytes. Journal of Cell Science, 123 (19), 3347, 2010.
  13. Sabeva N., Petkov N., Ogneva V.: Effect of Galega officinalis on glucose homeostasis in streptozotocin diabetic mice. Comp. rendus de l’Acad. bulgare des Sciences. 57, 95, 2004.
  14. Sybirna N. et al.: Sialic acid-containing glycoproteins are the marker molecules that determine the leukocytes functional state under diabetes mellitus. Sepsis. 4(1), 47, 2011.


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