Effect of combinational arginase and canavanine treatment on normal human peripheral blood lymphocytes in vitro
Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 4, Pages 385-389
Oleh Chen1,2, Bohdanna Kavalets1,2, Marina Barska1, Lilianna Lyniv1,
Olena Vovk1, Natalia Sybirna2, Oleh Stasyk1
1 Department of Cell Signaling, Institute of Cell Biology of NAS of Ukraine, Lviv, Ukraine
2 Department of Biochemistry, Ivan Franko Lviv National University, Lviv, Ukraine
Recombinant human arginase (rhARG)-based therapy to restrict arginine availability for malignant cells is a promising strategy to treat some cancers, including those of hematological origin. In the present study, we evaluated the action of combined rhARG treatment with canavanine (Cav) on proliferative potential and functional state of human peripheral blood lymphocytes (PBL) in vitro. We demonstrated that Cav in 0.1 mM concentration alone had no significant effect on proliferation and functional activity of normal activated PBL. Combined rhARG and Cav treatment inhibited proliferation and slightly promoted apoptosis in activated PBL but did not affect resting cells. However, the action of both drugs affected the ability of T cells for antigen activation. These results are important for further translational research on arginine-deprivation therapy.
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arginase, canavanine, arginine deprivation, peripheral blood lymphocytes
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