Current Issues of Pharmacy and Medical Sciences

Arginase/nitric oxide synthase regulatory mechanism of cells under the action of trigger factors that cause reactive arthritis

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 4, Pages 382-384

Oksana Melnyk, Olena Kornijchuk, Zinovy Vorobets, Mariya Tymkiv, Andriy Zakalskyy

Danylo Halytsky Lviv National Medical University, Ukraine


DOI: 10.12923/j.2084-980X/26.4/a.06

Abstract

On the basis of the research it can be argued that the determination of the enzymatic activity of arginase and NO-synthase of peripheral blood lymphocytes gives a qualitative informative assessment on the functioning of the immune cells, as changes in the activity of arginase reflect the NO metabolism. In the research, the optimal conditions of arginase reaction were determined and some of kinetic parameters of arginase were established. The significant growth of arginase activity (3.3 times) and iNO-synthase activity (3.8 times) in peripheral blood lymphocytes in patients with reactive arthritis compared with practically healthy donors was shown.

 

Keywords

arginase, L-arginine, NO-synthase, reactive arthritis, lymphocytes

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