Current Issues of Pharmacy and Medical Sciences

Comparison of agarose gel and capillary electro phoresis for the characterization of serum monoclonal paraproteins

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 3, Pages 299-304

MACIEJ KORPYSZ, ARLETA MALECHA-JĘDRASZEK, HELENA DONICA

Department of Biochemistry Diagnostics, Medical University of Lublin, Poland

DOI: 10.12923/j.2084-980X/26.3/a.13

 

Abstract

Serum protein electrophoresis is an especially useful method to detect and semi-quantify monoclonal proteins in patients with multiple myeloma and other plasmocyte dyscrasias. The presence of monoclonal protein (M protein) in electrophoretic separation is indicated by a sharp spike in gamma-globulin fraction that is sometimes located in alpha-2-globulins and beta-globulins. 
Semi-quantification of M protein is a basic method to monitor therapy of patients with multiple myeloma (MM) and monoclonal gammopathies of undetermined significance (MGUS). The purpose of the study was to compare concentrations of M protein obtained by agarose gel (AGE) and capillary electrophoresis (CE) and to evaluate diagnostic usefulness of both electrophoretic techniques for the identification of M protein. The investigations were carried out in the group of 90 patients with monoclonal gammopathies, 42 females and 48 males aged 65±9 years. Patients with monoclonal gammopathies had lower concentrations of monoclonal proteins determined by AGE in comparison to CE. High positive correlation between the results of monoclonal protein concentrations obtained by AGE and CE was observed. Both AGE and CE seem to be equally useful diagnostically in the detection of paraproteins.

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Keywords

monoclonal proteins (M protein), agarose gel electrophoresis (AGE), capillary electrophoresis (CE), monoclonal gammopathy

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