Current Issues of Pharmacy and Medical Sciences

Analysis of serum proteins by agarose gel and capillary electro phoresis

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 3, Pages 267-272

Maciej Korpysz, Arleta Malecha-Jędraszek, Helena Donica

Department of Biochemistry Diagnostics, Medical University of Lublin


DOI: 10.12923/j.2084-980X/26.3/a.05

Abstract

Electrophoresis is a basic technique used to identify disorders of blood serum protein fractions. Agarose gel is the most frequently  used medium for routine protein separations. However, capillary electrophoresis seems to be an attractive alternative to gel  electrophoresis. The article presents the results of comparative analysis of two systems (Sebia): Hydrasys designed for  electrophoretic separations on agarose gel and Minicap for capillary electrophoresis. The purpose of study was to evaluate  comparatively the concentrations of each serum protein fraction obtained by gel and capillary electrophoresis and to analyze the  correlations between the results obtained by those two systems depending on the concentrations of each protein fraction. The study  was carried out in the group of 98 patients, 46 females and 52 males. Despite slight quantitative differences in certain fractions  obtained by both methods, capillary electrophoresis offers a fully automatic process of analysis, high speed and efficiency which  proves that capillary electrophoresis is appropriate alternative to gel electrophoresis.

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Keywords

agarose gel electrophoresis (AGE), capillary electrophoresis (CE), Serum proteins

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