Current Issues of Pharmacy and Medical Sciences

Sterols and triterpenoids in selected plant species of the family Cyperaceae

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 2, Pages 215-220

MARCIN CZOP1, MAGDALENA JANYSZEK2, ANNA OCH3, MAREK OCH3, ANNA BOGUCKA-KOCKA3

Clinical Genetics Department, Medical University of Lublin, Poland
Department of Botany, University of Life Sciences, Poznań, Poland
Chair and Department of Pharmaceutical Botany, Medical University of Lublin, Poland

DOI: 10.12923/j.2084-980X/26.2/a.21

 

Abstract

Triterpenes and sterol fractions were isolated from the herb Scirpus maritimus L. and Carex Paradoxa Willd. by typical extraction with petroleum ether. The fraction of sterols was investigated us ing Gas Chromatography/Mass Spectrometry (GC/MS) techniques. The occurrence of cholesterol, campesterol, stigmasterol, and β-sitosterol 5α-stigmast-7EN-3α-ol has been observed in the Carex paradoxa Willd. Their percentage in the herb has been determined by quantitative analysis. The presence of campesterol, stigmasterol and a mixture of sitosterols in the herb Scirpus maritimus L. was indicated and its percentage in the herb was determined. Thin Layer Chromatography (TLC) was carried out to identify triterpenoids contained in investigated plants. By comparison with the standards of lupeol and β-amyrine the presence of lupeol and an unknown compound in Scirpus maritimus Willd. has been indicated. In the separation of triterpenoids Column Chromatography (CC) was used. Melting point of an unknown compound was marked, but it was unidentified because of lack of appropriate standards.

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Keywords

Scirpus maritimus L., Carex Paradoxa Willd., Sterols, Triterpenoids, GC-MS, TLC, CC

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