Current Issues of Pharmacy and Medical Sciences

Evaluation of the use of microcrystalline chitosan and collagen membranes as carriers for the platelet derived growth factor (PD

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 2, Pages 176-182

KAZIMIERA H. BODEK1, MARTA MICHALSKA2, ANDRZEJ BODEK3, MARCIN KOZAKIEWICZ4

1Department of Applied Pharmacy, Faculty of Pharmacy, Medical University of Lodz, Poland

2Department of Pharmaceutical Biochemistry, Faculty of Pharmacy, Medical University of Lodz, Poland

3Institute of Biopolymers and Chemical Fibres, Łódź, Poland

4 Department of Maxillofacial Surgery, Medical University of Lodz, Poland


DOI: 10.12923/j.2084-980X/26.2/a.13

Abstract

The aim of the present study was to evaluate the in vitro release of platelet-derived growth factor (PDGF-BB) from homogeneous microcrystalline chitosan (MCCh), collagen (Coll) and composite microcrystalline chitosan - collagen (MCCh-Coll) membranes in the presence or absence of amoxicillin (Am) and to select the most useful membrane for its practical application. The films were characterized by means of FTIR spectroscopy, swelling studies and SEM images. The kinetics of PDGF-BB release was evaluated by means of the ELISA immunoassay test. Amoxicillin concentration was determined spectrophotometrically at 273 nm. The process of the PDGF-BB growth factor and amoxicillin release from studied membranes was of two-phase nature. The first phase (during the 8 h) was characterized by rapid release whereas in the second phase (up to 10 days) the release was much slower. MCCh-Coll (M4) composite membrane appeared to be the most useful membrane because of the fast release of PDGF-BB as a significant angiogenic growth factor for tissue regeneration and the slow as well as gradual release of the antibiotic (amoxicillin), which carries protective role during regeneration process.

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Keywords

microcrystalline chitosan (MCCh), collagen (Coll), platelet derived growth factor (PDGF-BB), amoxicillin (Am), kinetics of PDGF-BB and Am release

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