Current Issues of Pharmacy and Medical Sciences

Determination of trace amounts of imazalil and thiabendazole in water- pipe “shisha” tobacco by UPLC-Q-TOF-MS

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 2, Pages 163-166

PAWEŁ SZPOT*, GRZEGORZ BUSZEWICZ

The Laboratory of Forensic Toxicology Medical University of Lublin, Poland

DOI: 10.12923/j.2084-980X/26.2/a.10

 

Abstract

The paper presents a method for determination of trace amounts of imazalil and thiabendazole in water-pipe tobacco. Fungicides were isolated with diisopropyl ether and analyzed by ultra-performance liquid chromatograph coupled with quadrupole – time of flight mass spectrometer (UPLC-Q-TOF-MS). The method for determination of imazalil and thiabendazole is highly sensitive. The  
lowest calibrated levels (LCL) were 0.2 ng/g and 10 ng/g respectively. Calibration curve was characterized by a very good linearity in the range for 0.2 – 100 ng/g for imazalil and 10 – 100 ng/g for thiabendazole (determination coefficient was 0.999 in both cases). The precisions (R.S.D.%) ranged from 1.56 % (imazalil) to 1.53 % (thiabendazole). Mean recovery was 129.44 % for imazalil and 84.73 % for thiabendazole. Usage of hybrid quadrupole coupled with time-of-flight analyzer characterized by a very high resolution allows to identify the investigated compounds and to apply the developed technique in routine quality control of fungicides in plant matrix.
 

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Keywords

tobacco, water-pipe, imazalil, thiabendazole, UPLC-Q-TOF-MS

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