Current Issues of Pharmacy and Medical Sciences

Examination of salivary proteins as biomarkers of pathological conditions. Literature review

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 1, Pages 71-75

ANNA SZYSZKOWSKA, JOANNA JAKIEL, JOANNA SZCZERBA, MANSUR RAHNAMA

Oral Surgery Department, Medical University of Lublin, Poland

DOI: 10.12923/j.2084-980X/26.1/a.16

 

Abstract

Saliva is universally available biofluid, easy to collect. Comprehensive analysis and identification of the proteomic content of human saliva may contribute to the understanding of oral pathophysiology and provide a foundation for the recognition of potential biomarkers of human disease. These features make it an ideal biological material for the early detection of many diseases of different origin, and enable non-invasive diagnostics. The presence of protein markers in saliva was found with usage of capillary electrophoresis and mass spectrometry.

Keywords

salivary proteins, salivary diagnostics, chromatography, mass spectrometry

References

  1. Al Kawas S., Rahim Z.H., Ferguson D.B.: Potential uses of human salivary protein and peptide analysis in the diagnosis of disease. Arch Oral Biol., 1,57,2012.
  2. Bradford M.M.: A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Bioch., 25,72,1976.
  3. Castagnola M. et al.: Biotechnological implications of the salivary proteome. Trends in Biotechnology 29,8,2011.
  4. de Jong E.P. et al: Sample collection and handling considerations for peptidomic studies in whole saliva; implications for biomarker discovery. Clin Chim Acta. 20,412,2011.
  5. Fitzgerald J. et al.: Immunoaffinity chromatography. Methods Mol. Biol., 681,2011.
  6. Gallant S.R., Koppaka V., Zecherle N.: Immunoaffinity chromatography.  Methods Mol. Biol., 421,2008.
  7. Giannobile W.V. et al.: Saliva as a diagnostic tool for periodontal disease: current state and future directions. Periodontology 2000., 50,52,2009.
  8. Gu S. et al: Global investigation of p53-induced apoptosis through quantitative proteomic profiling using comparative amino acid-coded tagging. Mol. Cell. Proteomics. 3,10,2004.
  9. Hagel L.: Gel filtration: size exclusion chromatography. Methods Biochem. Anal., 54,51,2011.
  10. Ho C.S. et al: Electrospray Ionisation Mass Spectrometry: Principles and Clinical Applications., Clin. Biochem. Rev. 24,1,2003.
  11. Huang C.M.: Comparative proteomic analysis of human whole saliva. Arch. Oral. Biol., 49,12,2004.
  12. Kaufmann M.: Unstable proteins: how to subject them to chromatographic separations for purification procedures. J. Chrom. B. Biomed. Sci. Ap., 10,699,1997.
  13. Laiko V.V., Baldwin M.A., Burlingame A.L.: Atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry. Anal. Chem., 72,4,2000.
  14. Lienqueo M.E. et al: Methodology for predicting the separation of proteins by hydrophobic interaction chromatography and its application to a cell extract. J. Chrom. A., 1009,1-2,2003.
  15. Lowry O.H. et al.: Protein measurement with the Folin phenol reagent. Biol.Chem., 193,1,1951.
  16. Moser A.C., Hage D.S.: Immunoaffinity chromatography: an introduction to applications and recent developments. Bioanalysis, 2,4,2010.
  17. Muronetz V.I., Sholukh M., Korpela T.: Use of protein – protein interactions in affinity chromatography. J. Biochem. Biophys. Methods, 49,1-3,2001.
  18. Nagler R.M.: Saliva as a tool for oral cancer diagnosis and prognosis. Oral Oncol., 45,12,2009.
  19. Queiroz J.A., Tomaz C.T., Cabral J.M.S.: Hydrophobic interaction chromatography of proteins. J. Biotech., 87,2,2001.
  20. Shevchenko A. et al: “De novo” sequencing of peptides recovered from in-gel digested proteins by nanoelectrospray tandem mass spectrometry. Mol. Biotech., 20,1,2002.
  21. Spielmann N., Wong D.T.: Saliva: diagnostics and therapeutic perspectives. Oral Diseases, 17,4,2011.
  22. Streckfus C.F. et al.: Breast cancer related proteins are present in saliva and are modulated secondary to ductal carcinoma in situ of the breast. Cancer Invest., 26,2,2008.
  23. Urh M., Simpson D., Zhao K.: Affinity chromatography: general methods. Methods Enzymol., 463,2009.
  24. Vitorino R. et al.: Identification of human whole saliva protein components using proteomics. Proteomics, 4,4,2004.
  25. Wilchek M., Chaiken I.: An overview of affinity chromatography. Methods Mol Biol.,147,2000.
  26. Wilmarth, P.A. et al: Two dimensional liquid chromatography study of the human whole saliva proteome. J. Prot. Res., 3,5,2004.
  27. Zhou Z. et al.: Ion-exchange-membrane-based enzyme micro-reactor coupled online with liquid chromatography-mass spectrometry for protein analysis. Anal Bioanal Chem., 403,1,2012.
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