Current Issues of Pharmacy and Medical Sciences

Determination of coumarins from aerial part of two Artemisia species

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 1, Pages 64-67

ALEKSANDRA JÓZEFCZYK, PIOTR PAWŁOWSKI

Chair and Dept. of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Poland

DOI: 10.12923/j.2084-980X/26.1/a.14

 

Abstract

The methanolic and methanolic-water extracts were received from a blooming aerial part (herb) of two species: Artemisia gmelinii Webb ex Stechmann and Artemisia umbelliformis Lam. Two methods were used for extracting the plant material: Accelerated Solvent Extraction (ASE) and Soxhlet Extraction. The analysis of samples was performed by the HPLC-DAD method. All obtained extracts were subjected to the process of the solid phase extraction. Samples for HPLC-DAD analysis were purified using C-18 (Octadecyl, 500 mg) Baker Bond (J.T. Baker, USA) SPE-micro-columns. The quantitative indications of individual constituents (coumarins) were converted into the content of scopoletin.

Keywords

Accelerated Solvent Extraction (ASE), Asteraceae, Artemisia gmelinii Webb ex Stechmann, Artemisia umbelliformis Lam., coumarins, HPLC-DAD analysis method

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