Current Issues of Pharmacy and Medical Sciences

Quantitative analysis of biofilm formation by oropharyngeal Candida albicans isolates under static conditions by confocal

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 1, Pages 54-56

Quantitative analysis of biofilm formation by oropharyngeal Candida albicans isolates under static conditions by confocal scanning laser microscopy


1Department of Pharmaceutical Microbiology, Medical University of Lublin, Poland
2Department of Comparative Anatomy and Anthropology, University of Maria Curie-Sklodowska, Poland

DOI: 10.12923/j.2084-980X/26.1/a.11



Candida albicans may colonize natural or artificial surfaces, leading to formation of the biofilm. Infections associated with the biofilm formation are important therapeutic problem. In this paper, we present data concerning the biofilm formation under static conditions by oropharyngeal isolates of C. albicans on a glass surface using confocal scanning laser microscopy (CSLM). The areal parameters describing the architecture of biofilm and its development, i.e. the areal porosity, the length of edge line, the length of skeleton line, were calculated. The changes in values of these parameters during the biofilm formation by C. albicans were similar for biofilm consisting of only blastospores as well as the biofilm consisting of blastospores and filamentous elements (hyphae or/and pseudohyphae). However, the thickness of C. albicans biofilm consisting of blastospores and filamentous elements was much higher than that consisting of only blastospores. The heterogeneity may be regarded as an important feature of the yeast biofilm including C. albicans.



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