Current Issues of Pharmacy and Medical Sciences

Effect of mobile phase buffer pH on separation selectivity of some isoquinoline alkaloids in reversed-phase systems of Pressuriz

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 1, Pages 45-49


1 Department of Physical Chemistry, Medical University of Lublin, Poland
2 Chair and Department of Medicinal Chemistry, Medical University of Lublin, Poland

DOI: 10.12923/j.2084-980X/26.1/a.09



Separation of some isoquinoline alkaloids (narcotine, chelidonine, dihydrocodeine, cinchonine, berberine, cinchonidine, papaverine, apomorphine) has been investigated with pressurized planar electrochromatography (PPEC) and high-performance thin-layer chromatography (HPTLC) in reversed-phase systems. The mobile phase consisted of acetonitrile and aqueous buffer (disodium phosphate and citric acid). The influence of the mobile phase buffer pH on migration distance (PPEC) and retardation factor (HPTLC) of the solutes has been investigated and compared. The results show different separation selectivity in both PPEC and HPTLC systems especially at pH range of buffer solution of the mobile phase that facilitates ionization of the solutes investigated.


pressurized planar electrochromatography, PPEC, high-performance thin-layer chromatography, HPTLC, isoquinoline


  1. Ashutosh Kar, (2008). Pharmacognosy & Pharmabiotechnolog. 2rd. New Age International (P) Limited, Publishers; p.372
  2. Chomicki A., Kloc K., Dzido T. H.: Two - Dimensional Separation of Some Amino Acids by HPTLC and Pressurized Planar Electrochromatography. J. Planar Chromatogr. 24, 6, 2011.
  3. Chomicki A., Ślązak P., Dzido T.H.: Preliminary results for 2-D separation with high-performance thin-layer chromatography and pressurized planar electrochromatography. Electrophoresis 30, 3718, 2009.
  4. Duncan J.D., Armstrong D.W., Chiral mobile phase additives in reversed-phase TLC. J. Planar Chromatogr. 3, 65-67, 1990.
  5. Dzido T.H., Plocharz P.W., Ślązak P.: Apparatus for Pressurized Planar Electrochromatography in a Completely Closed System. Anal. Chem. 78, 4713, 2006.
  6. Dzido T.H. Pressurized planar electrochromatography as the mode for determination of solvent composition-retention relationships in reversed-phase systems. J. Planar Chromatogr. 21, 295, 2008.
  7. Dzido T.H. Progress in planar electrochromatography. Anal. Bioanal. Chem. 391, 2111, 2008.
  8. Hałka A. Reversed-Phase Pressurized Planar Electrochromatography and Planar Chromatography of Acetylsalicylic Acid, Caffeine, and Acetaminophen. J. Planar Chromatogr. 23, 420, 2010.
  9. Hałka - Gryśniska A. Simultaneous determination of acetaminophen, propyphenazone and caffeine in cefalgin preparation by pressurized planar electrochromatography and high-performance thin-layer chromatography. Anal. Methods 4, 973, 2012
  10. Houghton P. J., Latiff A., Said I.M.: Alkaloids from Mitragyna speciosa. Phytochemistry 30, 347-350, 1991
  11. Houghton P.J., Osibogun I.M.: The use of silica gel coated with liquid paraffin as a stationary phase for chromatographic analysis of phytochemicals. Phytochem. Anal. 1, 22-25, 1990.
  12. Kopciał E., et. al.: The Effect of Mobile Phase Composition on Separation of Some Non-Steroidal Anti-Inflammatory Drugs of the 2-Arylpropanoic Acid Derivatives in System of Reversed-Phase Pressurized Planar Electrochromatography and High-Performance Thin-Layer Chromatography. Curr. Issues Pharm. Med. Sci. 25(3), 282-285, 2012.
  13. Mazak K., et. al.: Lipopholicity of vicopocetine and related compounds characterized by reversed-phase thin layer chromatography. J. Chromatogr. A 996,195-203, 2003
  14. Nurok D. Apparatus and Initial Results for Pressurized Planar Electrochromatography. Anal. Chem. 76, 1690, 2004.
  15. Petruczynik A., Waksmundzka-Hajnos M., Hajnos M.: The effect of chromatographic conditions on the separation of selected alkaloids in RP-HPTLC. J. Chromatogr. Sci. 43, 183-194, 2005
  16. Petruczynik A., TLC of alkaloids on cyanopropyl bonded stationary Phases. II. Connection with PR 18 and silica plates. J. Chromatogr. Sci. 46(4):, 291-7, 2008.
  17. Płocharz P.W., Klimek-Turek A., Dzido T.H.: Pressurized planar electrochromatography, high - performance thin - layer chromatography and high -performance liquid chromatography - Comparison of performance J. Chromatogr. A 1217, 4868, 2010.
  18. Płocharz P. W., Influence of sample application mode on performance of pressurized planar electrochromatography in completely closed system. Chromatogr. A 1170, 91-100, 2007.
  19. Polak B., Separation of Some Aromatic Amino Acid Enantiomers with Pressurized Planar Electrochromatography and TLC. Chromatographia 73, 339, 2011.
  20. Polak B., Hałka A., Dzido T.H.: Pressurized Planar Electrochromatographic Separationof the Enantiomers of Tryptophan and Valine. J. Planar Chromatogr. 21, 33, 2008.
  21. Shalby A., Khalil H.: Reversed phase ion-pair chromatography of some alkaloids. J.Liq. Chromatogr. 22, 2345-2352, 1999.
  22. Volkmann D.: Ion pair chromatography with UV absorbing counterions on reversed-phase layers. Part I. Chromatography with hydroxynaphtalenesulfonic acids. J. High Resol. Chromatogr. 6, 378-380, 1983.
  23. Waksmundzka-Hajnos M., Determination of the lipophilicity of selected isoquinoline alkaloid by RP- TLC. Acta Chromatogr. 20(4), 563 - 573, 2008.
prev next



April 2020

Mon Tue Wed Thu Fri Sat Sun
    01 02 03 04 05
06 07 08 09 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30