Current Issues of Pharmacy and Medical Sciences

Effect of mobile phase buffer pH on separation selectivity of some isoquinoline alkaloids in reversed-phase systems of Pressuriz

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 1, Pages 45-49


1 Department of Physical Chemistry, Medical University of Lublin, Poland
2 Chair and Department of Medicinal Chemistry, Medical University of Lublin, Poland

DOI: 10.12923/j.2084-980X/26.1/a.09



Separation of some isoquinoline alkaloids (narcotine, chelidonine, dihydrocodeine, cinchonine, berberine, cinchonidine, papaverine, apomorphine) has been investigated with pressurized planar electrochromatography (PPEC) and high-performance thin-layer chromatography (HPTLC) in reversed-phase systems. The mobile phase consisted of acetonitrile and aqueous buffer (disodium phosphate and citric acid). The influence of the mobile phase buffer pH on migration distance (PPEC) and retardation factor (HPTLC) of the solutes has been investigated and compared. The results show different separation selectivity in both PPEC and HPTLC systems especially at pH range of buffer solution of the mobile phase that facilitates ionization of the solutes investigated.


pressurized planar electrochromatography, PPEC, high-performance thin-layer chromatography, HPTLC, isoquinoline


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