Current Issues of Pharmacy and Medical Sciences

The analysis of flavonoids in the flowering herbs of Carduus acanthoides L.

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 1, Pages 10-15

MAŁGORZATA KOZYRA, KAZIMIERZ GŁOWNIAK, MAŁGORZATA BOGUSZEWSKA

Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Poland

DOI: 10.12923/j.2084-980X/26.1/a.02

 

Abstract

The present paper discusses the analysis of flavonoids in the methanol extract from the flowering herbs of Carduus acanthoides (L.), where flavonois were indentified by TLC and SPE HPLC. The quantitative analysis was performed using spectrophotometric Christ-Müller’s method. The samples containing flavonoids and those released after acid hydrolysis were investigated by 1D TLC on silica gel and polyamide. After purification by SPE, the samples were also analyzed by RP-HPLC. Apigenin-7-glucoside, luteolin-7-glucoside, kaempferol-3-rhamnoglucoside, kaempferol-3-glucoside, apigenin, luteolin and chlorogenic acid and p-coumaric acid were detected in the fractions of methanol extracts obtained from the plant. This method is very simple for the analysis and suitable for rapid screening of flavonoids in plants.

Keywords

Carduus acanthoides L., TLC, RP HPLC, flavonoids

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