Current Issues of Pharmacy and Medical Sciences

The analysis of flavonoids in the flowering herbs of Carduus acanthoides L.

Current Issues in Pharmacy and Medical Sciences Vol. 26, No. 1, Pages 10-15


Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Poland

DOI: 10.12923/j.2084-980X/26.1/a.02



The present paper discusses the analysis of flavonoids in the methanol extract from the flowering herbs of Carduus acanthoides (L.), where flavonois were indentified by TLC and SPE HPLC. The quantitative analysis was performed using spectrophotometric Christ-Müller’s method. The samples containing flavonoids and those released after acid hydrolysis were investigated by 1D TLC on silica gel and polyamide. After purification by SPE, the samples were also analyzed by RP-HPLC. Apigenin-7-glucoside, luteolin-7-glucoside, kaempferol-3-rhamnoglucoside, kaempferol-3-glucoside, apigenin, luteolin and chlorogenic acid and p-coumaric acid were detected in the fractions of methanol extracts obtained from the plant. This method is very simple for the analysis and suitable for rapid screening of flavonoids in plants.


Carduus acanthoides L., TLC, RP HPLC, flavonoids


  1. Bain J., Desrochers A.: Flavonoids of Carduus nutans and C. acanthoides; Biochem. Syst. Ecol., 16, 3, 265-268,1988.
  2. Cieśla Ł., Waksmundzka-Hajnos M.: Two-dimensional thin- layer chromatography in the analysis of secondary plant metabolites. J. Chrom. A., 1216, 7, 1035-1052, 2009.
  3. Coleta M., Campos M., Cotrimo M. et al.: Assessment of luteolin (3’,4’,5,7-tetrahydroflavone) neuropharmacological activity. Behav. Brain Res., 189, 75-82, 2008.
  4. Daszykowski M., Hawrył M., Waksmundzka-Hajnos M., Walczak M.: Identification of similar and orthogonal chromatographic Thin-Layer systems for two-dimentional separations of flavonoids and their analogues. Acta Chromatogr., 20, 3, 283-307, 2008.
  5. Polish Pharmacopoeia VI. Ed., Polskie Towarzystwo Farmaceutyczne, Warszawa, 150, 2002
  6. Fernandez I., Garcia B., Varea J.R., Varea A.: Lignans and Flavonoids from Carduus assoi. Phytochemistry, 30,3,1030-1032,1991.
  7. Frydman B., Deulofeu V.: Tetrahedron, argentine plants, XIX. Alkaloids from Carduus acanthoides. Structure of acanthoine and acanthoidine and synthesis of racemic acanthoidine. Elsevier, ISSN 0040-4020, ZDB-ID 2007072-X.,18,9,1063-1072, 1962.
  8. Hirano T., Higa S., Arimitsu J. et al.: Flavonoids such as luteolin, fisetin and apigenin are inhibitors of interleukin-4 and interleukin-13 production by activated human basophiles. Int. Arch Allergy Immunol., 134, 2, 135-40, 2004.
  9. Hwan Ch., Gan J., Jong al.: Blood glucose lowering and analgesic composition; Repub. Korean Kongkae Taeho, Kongbo, 2003, KR 20033064921 A 20030806.
  10. Jordon - Thaden I., Louda S.: Chemistry of Cirsium and Carduus: a role in ecological risk assessment for biological control of weeds. Biochem. Syst. Ecol., 31, 12, 1353-1396, 2003.
  11. Kaloshina N.A., Glyzin V.I., Bankowskii A.I.: Flavonoids from Carduus nutans; Khimiya Prirodnykh Soedinenii, 5, 654-655, 1975.
  12. Kaloshina N., Mazulin A.: Flavonoids of Carduus nutans; Khimiya Prirodnykh Soedinenii., 3, 453, 1988.
  13. EL-Lakany A.M., Abdel-Kader M., Maged S. et. al.: A new flavone glycoside with antimicrobial activity from Carduus pycnocephalus, Pharmazie, 52, 1, 78-79, 1997.
  14. EL-Lakany A.M., Abdel-Kader M., Maged S. et al.: Flavonoids from Carduus pycnocephalus L. J of Pharm. Sci., 9, 1, 41-43, 2005.
  15. Pietta P., Mauri P.: Analysis of flavonoids in medicinal plants. Methods Enzymol., 335, 26-45, 2001.
  16. Pietta P.G., Simonetti P. in: Packer L.: Antioxidant Food Supplements in Human Health, Academic Press, San Diego, 283-308, 1999.
  17. Robards K., Antolovich M.: Analytical chemistry of fruit bioflavonoids. Analyst., 122, 2, 11-34, 1997.
  18. Shirley B. W.: Flavonoid biosynthesis: ‘new’ functions for an ‘old’ pathway. Trends in Plant Science, 1,11, 377-382, 1996.
  19. Stalikas C.: Extraction, separation and detection methods for phenolic acids and flavonoids. J. Sep. Sci., 30, 18, 3268-3295, 2007.
  20. Terentjeva S., Krasnov E.: Coumarins and flavonoids of above-ground part of Carduus crispus L. and C. nutans L. Rastitel'nye Resursy, 39, 55-64, 2003.
  21. Zgórka G., Hajnos A.: The application of solid-phase extraction and reversed phase high-performance liquid chromatography for simultaneous isolation and determination of plant flavonoids and phenolic acids. Chromatographia, 57, 1, 77-80, 2003.
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