Current Issues of Pharmacy and Medical Sciences

The effect of protocatechuic acid on the tongue mucosa in rats exposed to n-nitrosomethylbenzylamine

Annales UMCS, Sectio DDD, Pharmacia, Vol. XXIV, N 4, 19

JUSTYNA SZUMIŁO1, ELŻBIETA STAROSŁAWSKA2, HELENA DONICA3,
MICHAŁ SZUMIŁO4, ROBERT KLEPACZ1, FRANCISZEK BURDAN2,5

1Department of Clinical Pathomorphology, Medical University of Lublin; Lublin, Poland

2St. John’s Cancer Center; Lublin, Poland

3Department of Biochemistry Diagnostics, Medical University of Lublin; Lublin, Poland

4Department of Applied Pharmacy, Medical University of Lublin; Lublin, Poland

5Department of Human Anatomy, Medical University of Lublin; Lublin, Poland

Abstract

The effect of natural plant-derived phenol – protocatechuic acid on lesions induced by carcinogen – N-nitrosomethylbenzylamine in the squamous epithelium of the tongue was investigated in rats. Carcinogen was applied for 3-8 weeks of the experiment (0.5 mg/kg/dose; 3 doses/week). Protocatechuic acid was administered with a daily diet in two doses 1000 or 2000 ppm for first 7 or 24 weeks of the experiment. Incidence and multiplicity of gross lesions on the dorsal surface of the tongue was the highest in rats exposed exclusively to carcinogen. Multiplicity of lesions was significantly lower in the group fed with diet containing protocatechuic acid in higher dose for the whole experiment. The most frequent lesion in the microscopic examination was hyperplasia of the squamous epithelium. Dysplasia and papillomas were rarely seen, while carcinomas were not observed. Chemoprevention activity of protocatechiuc acid in applied experimental model was confirmed.

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Keywords

protocatechuic acid, N-nitrosomethylbenzylamine, experimental carcinogenesis, tongue, rat

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