Current Issues of Pharmacy and Medical Sciences

Analysis of phenolic acids in hyssop (Hyssopus officinalis L.) by HPLC-DAD with monolithic column ChromolithTM RP-18e a)

Annales UMCS, Sectio DDD, Pharmacia, Vol. XXIV, N 4, 5

TOMASZ BAJ1, ANNA BŁAŻEWICZ2, ŁUKASZ ŚWIĄTEK3,
TADEUSZ WOLSKI1, RYSZARD KOCJAN2, KAZIMIERZ GŁOWNIAK1

1 Department of Pharmacognosy with Medicinal Plant Unit, Medical University in Lublin, Poland
2 Department of Analytical Chemistry, Medical University in Lublin, Poland
3 Department of Virology, Medical University in Lublin, Poland

Abstract

In the present study the ChromSword® software was used in order to optimize the chromatographic separation of selected phenolic acids in the monolithic stationary phase. A mobile phase gradient composed of a mixture of water, acetonitrile and acidic acid was used. The computer simulation of HPLC separation made obtaining optimal conditions of separation possible, as well as high linearity (linear correlation coefficient ranging from 99.1 to 99.9%), suggesting high accuracy of quantitative analysis. By using monolithic columns, less than 6 minutes were required to analyze seven phenolic acids. Very low limit of detection (LOD) values of phenolic acids, ranging from 1.9 to 7.9 mg/L, were achieved. The application of optimized mobile phase gradient for the analysis of phenolic acids occurring in Hyssopus officinalis L. allowed for confirmation of the presence of protocatechuic, gentisinic, chlorogenic, caffeic, ferulic, gallic and rosmarinic acids in this plant.

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Keywords

Chromolith, ChromSword, HPLC, hyssop, Hyssopus officinalis L., phenolic acids

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