Current Issues of Pharmacy and Medical Sciences

The GC/MS and HPLC/DAD Analysis of Phenolic Acids from Winter Savory (Satureja montana)

Annales UMCS, Sectio DDD, Pharmacia, Vol. XXIV, N 4, 4

JÓZEF RZEPA1, MIECZYSŁAW SAJEWICZ1, TOMASZ BAJ2,
PATRYCJA GORCZYCA1, MAGDALENA WŁODAREK1,
STANISŁAW KWIATKOWSKI2, TERESA KOWALSKA1,
MONIKA WAKSMUNDZKA-HAJNOS3

1 Institute of Chemistry, University of Silesia, Katowice, Poland
2 Department of Pharmacognosy with Medicinal Plant Unit, Medical University in Lublin
3 Department of Inorganic Chemistry, Medical University in Lublin

Abstract

An effort was undertaken to identify phenolic acids contained in the water sample remaining from steam distillation of the volatile fraction contained in winter savory (Satureja montana). Solid phase extraction (SPE) was employed to isolate the phenolics from water. Additionally, the accelerated solvent extraction (ASE) was used to isolate the phenolics from the dried winter savory material. The phenolics derived both from the water residue and those obtained with use of ASE were first derivatized and then analyzed by means of GC/MS. The non-derivatized phenolics originating from ASE were fingerprinted and semi-quantitatively assessed by means of HPLC/DAD. A comparison was made of the GC and HPLC results, and the conclusions were drawn as to the identified compounds, depending on the applied analytical approach. Usefulness of the complementary approach was shown, as each of these two techniques allowed detection of the different phenolic acids.

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Keywords

savory, Satureja montana, phenolic acids, SPE, ASE, HPLC/DAD

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