Application of isobolographic analysis for the evaluation of interactions between bioactive constituents from dandelion ...
Annales UMCS, Sectio DDD, Pharmacia, Vol. XXIV, N 3, 17
Department of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, Poland
Reactive oxygen species (ROS) have been implicated in more than 100 diseases. In living organism ROS can be formed eg.with lipoxygenase (LOX) action. In this study the LOX-inhibitory activity and phenolics profiles of dandelion and lime flowers tinctures were compared and the interactions between them were evaluated using an isobolographic analysis. Dandelion flowers tinctures were a good source of phenolic acids, whereas lime flowers tinctures were better source of quercetin, kaempferol and apigenin. Higher LOX inhibitory activity was obtained for dandelion tincture (IC50 about 36.4 mg d.m. /ml) whereas for lime tincture IC50 average about 46.4 mg d.m./ml. Isobole curve for 50% inhibition of dandelion : lime flowers mixture took the convex form. This result indicates that LOX inhibitors of studied preparation acted antagonistically.
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Isobolic analysis, dandelion, lime, phenolic compounds, lipoxygenase