Current Issues of Pharmacy and Medical Sciences

A simple, rapid capillary electrophoretic (CE) method has been established and validated for determination of antiepileptic drug lamotrigine, pure substance and in tablets. The method was developed by employing fused silica capillary with an effective length of 80 cm and internal diameter of 75 μm. 0.067 M phosphate buffer with pH 4.80 was used for lamotrigine analysis. The recommended applied voltage, capillary temperature and injection time were 30 kV, 30°C and 10 s, respectively. Detection was followed by direct absorptiometric measurements at 210 nm. Developed method was linear in the range of 5-100 μg/mL and had good correlation coefficient 0.9996. Intra and inter-day precision, calculated as relative standard deviation (RSD), were better than 2.5 %. The proposed CE method was successfully applied to the assay of lamotrigine in pharmaceutical formulation. Excipients present in the tablets did not interfere in the assay.